BIO115 San José State University Biology Lab 2 Worksheet Biology Lab report Answer the following questions …….. F G H к Enter B N M Shift Instructor: B

BIO115 San José State University Biology Lab 2 Worksheet Biology Lab report Answer the following questions …….. F
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Instructor:
Biology 115 Lab Fall 2018
Lab 2 Worksheet
2. What would happen if we switched the positive and negative electrode on the gel box? (1pt]
3. How large is the banana genome? If we tried to run the DNA you extracted from the banana
today on the gel where would we expect to see the band? [1pt]
4. Look at your gel picture. Why does lambda DNA contain multiple bands while the plasmid
lanes only have a couple? [1pt]
Total Score:
10.00
(Includes
Comments:
Bonus Points for
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Biology 115 Lab Fall 2018
Lab 2 Worksheet
Instructor:
[2 pts]
V. Observations & Results
VA. Data
Observations of extracted DNA
Agarose Gel FULLY labeled (ladder, lanes, lengths)
VI. Discussion Questions
1. Give a reason (and use your data for an example) of how even though 2 pieces of DNA are the
exact same length, they do not travel an equal distance on an agarose gel. [2pt]
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Biology 115 Lab Fall 2018
Lab 2 Worksheet
Instructor:
1. Student Name:
Group Members:
II. Personalized Title
III. Hypothesis
[1.0 pts)
1.
IV. Introduction
IVA. General Theory/Background for both parts of the experiment. 250 word minimum. Do not copy
and paste from lab manual – this will result in a 0 for the entire lab report. [2.0 pts]
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7. Insert a coffee filter into a clean beaker leaving “1 inch of space from bottom of
beaker
8. Secure the filter over the rim of the beaker with a rubber band.
9. Add 25 mL of banana solution to the filter.
10. Wait 10 minutes. If the filtered volume is less than 5mL add more solution. Do not
tear filter
Remove Proteins from Lysate
1. Pipet 1mL of protease into a test tube
2. Add 5mL of filtered banana solution to the test tube. Gently mix.
3. Leave at room temperature for 3min
Precipitate the DNA
1. Tilt the falcon tube containing 15mL of ice cold isopropyl alcohol.
2. Slowly pipet the pineapple-banana solution so the mixture forms a layer on top of
the isopropyl alcohol.
3. DO NOT MIX!
4. Incubate the test tube at room temperature for 10 min until the DNA begins to
precipitate
5. Inset a loop into the tube and slowly spool the DNA onto the rod.
2. Load your samples into the gel in the following order:
Lane:
1: 15ul Tube 1
2: 15ul Tube 2
3: 15ul Tube 3
4: 15ul negative control
3. When you are finished loading, place the cover on the gel and connect the electrodes.
4. The gels are run for at least 30 minutes at 125 V, or until the bands have reached at least 3/4
down the gel.
5. When electrophoresis is complete, we will visualize a gel on the trans-illuminator. Each group
will take pictures of their gel using the Eagle-Eye photo system. This gel picture should
be labeled, analyzed, and included in your lab report.
DNA Extraction
(Done Before Lab) Preparation of Detergent
1. Dissolve 3g of NaCl (Table Salt) in 80 mL of diH20
2. Add 10 mL of liquid detergent (with EDTA). Add diH2O to final volume of 100mL
Prepare the Lysate
1. Mash 100g of banana with mortar and pestle until smooth.
2. Place in 250ml beaker
3. Add 100mL of detergent solution
4. Mix gently and thoroughly
5. Place beaker in 60 degree C water bath for 15 min. Mix frequently
6. Move beaker to ice bath for 5 min to cool.

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